ABSTRACT
Objective To study the effects of calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of(c-junmRNA) and protein in striate cortex of rats with transient global cerebral ischmia/reperfusion. Methods In situ hybridization,immunohistochemistry and microscope image analysis were used. Results The expression of c-jun mRNA in ischemia/reperfusion(I/R) group was increased as compared with sham group(P
ABSTRACT
Objective: Our purpose was to explore the protective role of Salvia Miltiorrhiza (SM) on gentamicin (GM) ototoxicity. Methods: We used NADPH-diaphorase in histochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement to investigate the change of nitric oxide synthase (NOS) activity in cochlea of guinea pig after the injection of GM and SM. Results: Gebtamicin and SM significantly reduced NOS activity in cochlear and the threshold of ABR compared with GM alone (P<0.01); and the threshold of ABR was in high correlation with NOS activity (rGM= －0.8236 ～ －0.8662; rSM+GM= －0.8628 ～ －0.9172, P<0.01). Conclusion: Salvia Miltiorrhiza can reduce NOS activity in cochlea, alleviate GM ototoxicity, and ameliorate hearing function.
ABSTRACT
The distribution of neurokinin B (NKB) in the lower respiratory tract of 16 guinea pigs was investigated using radioimmunoassay. As a result, it is noted that the concentration of NKB is the highest in the lung (43.83?4.37), moderate in the bronchus (36.12?5.36) and lowest in the trachea (26.44?3.73 pg/g wet tissue). NKB-immunoreactive fibers were demonstrated by immunohistochemistry in the following locations of the lower respiratory tract: 1. within and beneath the lining epithelium, smooth muscle layer and tunica adventitia of the trachea and bronchus; 2. in the smooth muscle layer of the bronchus in the lung; 3. in the alveolar septurn; 4. within smooth muscle layer and the border between smooth muscle and tunica adventitia of the blood vessels and around the blood vessels in the lung. It is also discussed that there may be a significance about the results for exploring the function of NKB and its relationship with some respiratory diseases.
ABSTRACT
Immunohistochemical study revealed the distributions of substance P, neurokinin A, neurokinin B and calcitonin gene related peptide immunoreactive nerve fibers in the medulla, cortex and capsule of tracheobronchial lymph nodes in 10 guinea pigs. More immunopositive fibers were found to be distributed in the area around the blood vessels and lymph sinuses, less in the area distant to blood vessels. The four kinds of positive immunoreactive nerve fibers were also seen within the wall of the blood vessels. The results provide some morphological basis for the study of neuroimmunomodulation.